We can sequence double-stranded and single-stranded templates from from purified plasmids, BACs, cosmids, and PCR products. Please see our DNA Prep/Concentration page for information on DNA sample submission and concentration.
Currently, we use two ABI 3730 Capillary Electrophoresis Genetic Analyzers for sequencing. These instruments are capable of processing approximately twelve 96-well plates in a 24-hour period. We use ABI BigDye Terminator v3.1 Cycle Sequencing chemistry which, together with our instruments, enable us to frequently achieve read lengths of 800-900+bp.
We also offer a run-only fragment analysis service on our ABI 3130XL Capillary Electrophoresis Genetic Analyzer. Click here for more information and instructions on starting a new project with us.
NGS and PacBio
If you are interested in Illumina and/or PacBio sequencing, please contact the UC Davis Genome Center DNA Technologies and Expression Analysis Cores.
**COVID-19 Update Jan 25 2021**
California's Statewide December stay-at-home order does not affect UC Davis Phase 2X research so UCDNA Sequencing will continue its normal services.
UCDNA Sequencing is available to receive DNA sequencing samples and fragment analysis runs during specific drop-off times on specific days. Runs and turn-around time on data will vary, depending on sample volume. Current turn-around time is approximately 2-3 days.
DNA Sample Drop-Off Schedule:
Storer Hall: Mon, Wed & Fri until 12pm
VM3B Annex: Mon, Wed & Fri until 11am
UCDMC: By request only
If this schedule does not work for you, please contact us 530-754-9259 and we will arrange another time for you to drop off your samples.
Sample Drop-off Instructions:
Storer Hall is locked and requires authorized access and COVID testing to enter the building so we have provided a FedEx envelope taped to the front doors of Storer Hall for sample drop-off. Place your samples in a clean Ziploc with your order number on it, drop them off in the envelope during our scheduled drop-off times and email DNAdivas@ucdavis.edu that they are there.
The VM3B annex appears to be unlocked now so you may drop samples off on any day of the week. We will pick up samples on Mondays, Wednesdays and Fridays at 11am.
If you have a 96-well plate or fragment analysis samples, you will need to schedule a time to drop off to us in person on Monday, Wednesday or Friday at Storer Hall. We will arrange to meet you at the front or back door of Storer Hall to receive your samples. Please wear your PPE, including mask and gloves.
To minimize any exposure, please exercise caution and refrain from placing orders if you or anyone in your lab has recently been ill.
Shipping Instructions :
Our receiving department is open M-F so you can ship to us any day during the week. Please email DNAdivas@ucdavis.edu to let us know how many samples you will be sending and when.
Thank you from the UCDNA Sequencing Staff