We can sequence double-stranded and single-stranded templates from from purified plasmids, BACs, cosmids, and PCR products. Please see our DNA Prep/Concentration page for information on DNA sample submission and concentration.
Currently, we use two ABI 3730 Capillary Electrophoresis Genetic Analyzers for sequencing. These instruments are capable of processing approximately twelve 96-well plates in a 24-hour period. We use ABI BigDye Terminator v3.1 Cycle Sequencing chemistry which, together with our instruments, enable us to frequently achieve read lengths of 800-900+bp.
We also offer a run-only fragment analysis service on our ABI 3130XL Capillary Electrophoresis Genetic Analyzer. Click here for more information and instructions on starting a new project with us.
NGS and PacBio
If you are interested in Illumina and/or PacBio sequencing, please contact the UC Davis Genome Center DNA Technologies and Expression Analysis Cores.