DNA Concentration

We recommend that you not only UV spec your samples but also run them on a gel to double check your concentrations.

Plasmids: Because concentration measures the total amount of DNA, without accounting for the size, as the plasmid size increases so must your DNA concentration. A general guideline for double stranded plasmid templates are as follows:

Template Size: Concentration:
Double stranded 3kb
100-200ng/uL
Double stranded 4kb
200-250ng/uL
Double stranded 5kb
250-300ng/uL
Double stranded 6kb
300-400ng/uL
Double stranded 7kb
400-500ng/uL
Double stranded 8kb
500-700ng/uL
Double stranded 9kb
700-800ng/uL
Double stranded 10kb
800ng/uL-1ug/uL
Double stranded >10kb
1ug/uL++

PCR products: Please supply us with 2ng/uL per 100bp of PCR product. (Example: A 700bp PCR product would be submitted at 14ng/uL).

 

 
 
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